C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 7/00 (2006.01) A61K 39/12 (2006.01) C07K 14/08 (2006.01) C12N 7/02 (2006.01) C12N 7/04 (2006.01) C12N 7/08 (2006.01) C12N 15/40 (2006.01) G01N 33/543 (2006.01)
Patent
CA 2424400
The invention relates to the field of virology , more in particular to the field of vaccine production, more specifically to the in vitro propagation of virus, more specifically to the adaptation of virus to a cell line. The invention provides a method to determine the capability of an arterivirus to replicate in a green monkey cell line, comprising determining the amino acids at positions, which correspond to the amino acid positions 75-107 of GP2a of PRRSV isolate I-1102, more specifically amino acid position 88 and/or amino acid position 95 of said GP2a. The invention further discloses a method to produce arterivirus in green monkey cell line wherein the virulence of said arterivirus is maintained, with increased virus yield. The invention further provides a method to determine the attenuation of an arterivirus, comprising determining the amino acids at positions, which correspond to the amino acid positions 121-148 of GP5 of PRRSV isolate I- 1102, more specifically amino acid 136 of said GP5 and/or determining the amino acids at positions, which correspond to the amino acid positions 651-675 and/or amino acid positions 2331-2355 of ORF1ab of PRRSV isolate I-1102, more specifically amino acid 663 and/or 2343 of said ORF1ab. The invention also provides a method to attenuate the virulence of said arterivirus by changing said amino acids.
Kroese Michiel Valentijn
Meulenberg Johanna Jacoba Maria
Van Rijn Petrus Antonius
Boehringer Ingelheim Vetmedica Gmbh
Borden Ladner Gervais Llp
Id-Lelystad Instituut Voor Dierhouderij En Diergezondheid B.v.
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