Adapter-directed display system

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/63 (2006.01) C07K 17/00 (2006.01) C12N 15/10 (2006.01) C12Q 1/02 (2006.01)

Patent

CA 2465178

The present invention provides adapter-directed display systems for expressing exogenous polypeptide within a host cell and/or displaying the exogenous polypeptide on the outer surface of a genetic package. This subject systems are particularly useful for displaying a genetically diverse repertoire of monomeric and multimeric polypeptides. The invention also provides both expression and helper vectors and kits containing components of the subject display systems. Also provided are genetic packages displaying the exogenous polypeptides of particular interest. Further provided by the invention are methods of using the subject display systems.

La présente invention a trait à des systèmes de présentation dirigée vers des adaptateurs, permettant d'exprimer un polypeptide exogène à l'intérieur d'une cellule hôte et/ou de présenter le polypeptide exogène sur la surface externe d'un matériel génétique. Les systèmes selon l'invention sont particulièrement utiles pour présenter un répertoire génétiquement varié de polypeptides monomériques et multimériques. L'invention concerne également des vecteurs d'expression et auxiliaires et des trousses contenant des éléments des systèmes de présentation en question. L'invention se rapporte aussi à des matériels génétiques présentant les polypeptides exogènes d'intérêt. L'invention concerne en outre des procédés d'utilisation des systèmes de présentation en question.

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