G - Physics – 02 – B
Patent
G - Physics
02
B
88/103, 88/105,
G02B 17/08 (2006.01) G02B 21/00 (2006.01) G02B 21/14 (2006.01) G02B 21/16 (2006.01)
Patent
CA 2015228
The present invention concerns an adapter to be coupled to an objective lens of a microscope comprising a body member having a first coupling means at one end thereof to receive an objective lens of a microscope and having a second coupling means at the other end thereof to receive a microscope component. The body member has an axial light path running longitudinally therethrough and has a radial light path which intersects the axial light path. A source of light is disposed in the radial light path. A first filter is disposed in the radial light path downstream of the source of light for passing light at wavelengths which excite fluorescence in a specimen to be examined in a microscope and to remove light at wavelengths where visible fluorescence emission occurs in the specimen. A mirror is disposed in the intersection of the axial light path and radial light path. The mirror passes light through the axial light path and reflects light at excitation wavelengths, but does not reflect light at emission wavelengths. A second filter is disposed in the axial light path and is disposed above the mirror and adjacent the second coupling means. The second filter blocks excitation wavelengths which pass through the mirror. The adapter can easily be attached and detached to a conventional microscope. The adapter can provide fluorescence microscopy capability to a conventional microscope which was not designed for fluorescence microscopy.
Rathbone Richard Rodion
Wardlaw Stephen Clark
Borden Ladner Gervais Llp
Yale University
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