Adaptive baseline algorithm for quantitative pcr

Details Adaptive baseline algorithm for quantitative pcr Adaptive baseline algorithm for quantitative pcr

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C07H 19/00 (2006.01) C07H 21/02 (2006.01) C07H 21/04 (2006.01) C12P 19/34 (2006.01) G06F 19/00 (2006.01)

Patent

CA 2462823

The invention relates to baseline subtraction algorithms developed to reduce tube-to-tube and cycle-to-cycle variabilities during real time PCR amplification. Particularly, the invention relates to algorithms for determining the threshold cycle for the first reliable detection of the amplified nucleic acid product. The invention also relates to computer programs comprising the algorithms and methods and/systems implementing the algorithms.

Cette invention concerne des algorithmes de soustraction de ligne de base mis au point pour réduire des variabilités tube à tube ou cycle à cycle pendant une amplification PCR en temps réel. Elle concerne en particulier des algorithmes qui permettent de déterminer le cycle seuil pour la première détection fiable du produit d'acide nucléique amplifié. L'invention concerne en outre des programmes d'ordinateur comprenant les algorithmes et les procédés de l'invention, ainsi que les systèmes mettant en oeuvre ces algorithmes.

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