Adaptor molecules for dna and their application to synthesis...

C - Chemistry – Metallurgy – 12 – N

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195/1.14, 195/1.

C12N 15/00 (2006.01) C07K 14/62 (2006.01) C12N 1/20 (2006.01) C12N 15/10 (2006.01) C12N 15/66 (2006.01) C12N 15/70 (2006.01)

Patent

CA 1202581

TITLE ADAPTOR MOLECULES FOR DNA AND THEIR APPLICATION TO SYNTHESIS OF GENE-DERIVED PRODUCTS INVENTORS Saran A. Narang Ray J. Wu ABSTRACT OF THE DISCLOSURE Adaptor molecules have been prepared to comprise either start or stop signals for protein synthesis, in addition to recognition sites for restriction endo- nucleases. Separate adaptors may be used in a symmetrical duplex form. The start adaptor may include nucleotide base inserts to provide the correct reading frame of the triplet code in a DNA sequence with inappropriate reading frame. Insulin A-chain and B-chain genes of the human type, have been synthesized with the appropriate adaptor molecules provided on each end. The adapted DNA genes have been joined to replicable cloning vehicles and the hybrid DNA transferred to a host cell. The transformed host cell has been shown to contain the desired insulin gene.

375495

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