Adaptors, and synthesis and cloning of proinsulin genes

Details Adaptors, and synthesis and cloning of proinsulin genes Adaptors, and synthesis and cloning of proinsulin genes

C - Chemistry, Metallurgy

12

N

195/1.26

C12N 15/00 (2006.01) C07K 14/62 (2006.01) C12N 15/66 (2006.01)

Patent

CA 1204682

TITLE ADAPTORS, AND SYNTHESIS AND CLONING OF PROINSULIN GENES INVENTORS SARAN A. NARANG RAY J. WU ABSTRACT OF THE DISCLOSURE A human-like proinsulin gene and its analogs, have been synthesized by a combination of chemical and enzymatic methods. A number of different human-like proinsulin gene analogs with altered C-chains have also been designed and can be readily constructed as described. As a part of the strategy, an adaptor for trimming DNA has been designed, synthesized and used to recover the A-chain insulin gene with the desired sequence from a hybrid plasmid; a related adaptor for trimming DNA has been designed to shorten the C-chain gene or any gene. The synthetic proinsulin gene has been joined to a replicable cloning vehicle and the hybrid DNA transferred to a host cell. The transformed host cell has been shown to contain the desired human-like proinsulin gene.

404721

Affiliated with

Also associated with

Rating

Adaptors, and synthesis and cloning of proinsulin genes does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Adaptors, and synthesis and cloning of proinsulin genes, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Adaptors, and synthesis and cloning of proinsulin genes will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFCA-PAI-O-1266022