Adeno-associated viral (aav) liposomes and methods related...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/88 (2006.01) A61K 9/127 (2006.01) A61K 31/70 (2006.01) A61K 35/12 (2006.01) A61K 48/00 (2006.01) C12N 5/10 (2006.01) C12N 15/11 (2006.01) C12N 15/12 (2006.01) C12N 15/86 (2006.01)

Patent

CA 2171318

A composition for genetic manipulation which comprises a liposome comprised of lipid material, and adeno-associated viral (AAV) material. Typically, the AAV material is plasmid, and comprises a terminal repeat of the AAV genome. Methods are disclosed for introducing genetic material into cells by use of AAV liposomes. Accordingly, genetic material was introduced and integrated into stem cells, T cells, primary tumor cells, or tumor cell lines.

Composition servant aux manipulations génétiques comportant un liposome contenant un matériel liposomique et un matériel viral adéno-associé (AAV). Le matériel AAV peut être un plasmide comprenant une répétition terminale du génome de l'AVV. Sont également présentées des méthodes d'introduction de matériel génétique dans des cellules au moyen de liposomes p. ex. dans des cellules souches, des cellules T, des cellules de tumeurs primaires et des lignées de cellules tumorales.

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