Adenoviral e1a/e1b complementing cell line

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 5/10 (2006.01) C12N 7/01 (2006.01) C12N 7/02 (2006.01) C12N 15/861 (2006.01) C12N 5/08 (2006.01) C12N 15/10 (2006.01) C12N 15/33 (2006.01) C12N 15/34 (2006.01) C12N 15/63 (2006.01) C12N 15/64 (2006.01) C12N 15/861 (2006.01)

Patent

CA 2531559

Adenovirus packaging cell lines for growth of E1A/E1B deficient adenovirus that is substantially free of replication competent adenovirus (RCA), are provided. Methods for producing adenovirus substantially free of RCA are also provided, wherein the adenovirus is grown in a cell line containing coding sequences for adenovirus E1A and E1B, are operably linked to promoters that lack polynucleotide sequences sharing substantial sequence identity with the native adenovirus E1A and E1B promoters.

L'invention concerne des lignées cellulaires d'empaquetage d'adénovirus destinées à l'obtention d'adénovirus déficients E1A/E1B sensiblement exempts d'adénovirus capables de se répliquer (RCA). L'invention concerne également des procédés permettant de produire un adénovirus sensiblement exempt de RCA, consistant à cultiver un adénovirus dans une lignée cellulaire contenant des séquences de codage pour les adénovirus E1A et E1B qui sont couplées fonctionnellement à des promoteurs qui ne comprennent pas de séquences polynucléotidiques partageant une grande partie de la séquence d'identité avec les promoteurs des adénovirus E1A et E1B natifs.

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