Adenovirus-transfected primary cells and methods of pathway...

Details Adenovirus-transfected primary cells and methods of pathway... Adenovirus-transfected primary cells and methods of pathway...

C - Chemistry, Metallurgy

12

N

C12N 15/861 (2006.01)

Patent

CA 2547256

A primary cell culture is transfected with a vector comprising a reporter gene operatively linked to a cis-element. Expression of a candidate regulatory protein is induced in the cell culture and its effects on the cis-element are assayed.

L'invention concerne la transfection d'une culture de cellules primaires au moyen d'un vecteur comprenant un gène rapporteur lié de manière fonctionnelle à un élément cis. L'expression d'une protéine régulatrice candidate est induite dans la culture de cellules et les effets de celle-ci sur l'élément cis sont analysés.

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