Adipose derived adult stem cells in hepatic regeneration

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 5/00 (2006.01) C12N 15/67 (2006.01) C12P 21/00 (2006.01) C12N 5/06 (2006.01) C12N 5/08 (2006.01)

Patent

CA 2650594

The present invention provides a method to derive hepatic stem cells from stem cells derived from non- liver tissue. In one embodiment of the invention, hepatic stem cells are derived from adipose stem cells. The invention also provides a method of enhancing hepatic cytokine production (e.g., HGF) from ASCs, which may be useful in the regeneration of liver tissue when transplanted in vivo. Tissue culture conditions, including media conditions, are provided.

La présente invention concerne un procédé permettant de dériver des cellules souches hépatiques de cellules souches issues d'un tissu non hépatique. Dans un mode de réalisation de l'invention, les cellules souches hépatiques sont dérivées de cellules souches adipeuses. L'invention concerne également un procédé permettant d'augmenter la production de cytokine hépatique (par exemple, le facteur de croissance hépatocytaire - HGF) à partir de cellules souches adipeuses (CSA), laquelle peut être utile dans la régénération du tissu hépatique lors d'une transplantation in vivo. L'invention concerne aussi les conditions de culture tissulaire, y compris les conditions relatives aux milieux.

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