C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/00 (2006.01) C12N 5/077 (2010.01) C12N 5/0775 (2010.01) A61K 35/12 (2006.01) A61K 48/00 (2006.01) C12N 11/00 (2006.01) C12N 11/08 (2006.01) C12N 15/09 (2006.01) C12N 15/85 (2006.01) C12N 15/87 (2006.01)
Patent
CA 2366078
The present invention provides adipose-derived stem cells and lattices. In one aspect, the present invention provides a lipo-derived stem cell substantially free of adipocytes and red blood cells and clonal populations of connective tissue stem cells. The cells can be employed, alone or within biologically-compatible compositions, to generate differentiated tissues and structures, both in vivo and in vitro. Additionally, the cells can be expanded and cultured to produce hormones and to provide conditioned culture media for supporting the growth and expansion of other cell populations. In another aspect, the present invention provides a lipo-derived lattice substantially devoid of cells, which includes extracellular matrix material from adipose tissue. The lattice can be used as a substrate to facilitate the growth and differention of cells, whether in vivo or in vitro, into anlagen or even mature tissues or structures.
La présente invention concerne des cellules souches et des réseaux dérivés de tissus adipeux. Dans un aspect, la présente invention concerne une cellule souche lipo-dérivée sensiblement exempte d'adipocytes et d'érythrocytes et de populations clonales de cellules souches de tissu conjonctif. On peut utiliser les cellules, seules ou dans des compositions biologiquement compatibles, pour créer des tissus et structures différenciés à la fois in vivo et in vitro. En outre, on peut mettre en expansion et en culture les cellules pour produire des hormones et créer des milieux de culture conditionnés destinés à favoriser la croissance et l'expansion d'autres populations de cellules. Dans un autre aspect, la présente invention concerne un réseau lipo-dérivé sensiblement exempt de cellules et comprenant une matrice extracellulaire provenant de tissus adipeux. On peut utiliser le réseau comme substrat pour faciliter la croissance et la différenciation des cellules, in vivo ou in vitro, dans un primordium ou même dans des tissus ou structures mûrs.
Benhaim Prosper
Futrell J. William
Hedrick Marc H.
Katz Adam J.
Llull Ramon
R. William Wray & Associates
The Regents Of The University Of California
University Of Pittsburgh Of The Commonwealth System Of Higher Ed
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