Adrenergic receptor snp for improved milking characteristics

C - Chemistry – Metallurgy – 07 – H

Patent

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C07H 21/02 (2006.01) C07H 21/04 (2006.01) C12P 19/34 (2006.01)

Patent

CA 2539551

Disclosed herein is a method for screening for the allele associated with a desired SCS phenotype, which comprises: obtaining a DNA sample from a bull to be tested for the desired SCS phenotype; and detecting the presence of an adenine at position 11 in a gene encoding a bovine beta2-adrenoreceptor. Also disclosed is a milking attribute PCR-RFLP kit containing a pair of primers which flank the 11th nucleotide position of the bovine beta2-adrenoreceptor gene, and a restriction enzyme specific for the CCCGGG site, which can be SmaI.

L'invention concerne un procédé pour dépister l'allèle qui est associé à un phénotype SCS souhaité, consistant : à obtenir un échantillon d'ADN d'un taureau devant être soumis au dépistage du phénotype SCS souhaité, et ; à détecter la présence d'une adénine au niveau de la position 11 d'un gène codant un bêta2-adrénorécepteur bovin. Cette invention concerne en outre un nécessaire d'attributs de traite PCR-RFLP contenant une paire d'amorces qui s'accolent à côté de la 11?ème¿ position nucléotidique du gène codant un bêta2-adrénorécepteur bovin, ainsi qu'une enzyme de restriction qui est spécifique du site CCCGGG, et qui peut être l'enzyme SmaI.

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