B - Operations – Transporting – 01 – J
Patent
B - Operations, Transporting
01
J
B01J 20/24 (2006.01) C07K 1/22 (2006.01) C07K 14/78 (2006.01)
Patent
CA 2083168
The invention provides a selective adsorbent for cellular fibronectin (cFN) and a method for fractional purification of FN which comprises contacting an FN material containing plasma fibronectin (pFN) and cFN with a cross linked polysaccharide sulfate and/or an immobilized polysaccharide sulfate to fractionate the pFN and cFN. By the fractional purification method of the invention, cFN and pFN can be fractionated in an expedient manner and with high efficiency and both pFN and cFN can be recovered in high purity and good yield.
L'invention concerne un adsorbant sélectif de la fibronectine cellulaire (cFN), ainsi qu'une méthode de purification par fragmentation de la FN, consistant à mettre en contact de la FN constituée de fibronectine plasmatique (pFN) et de cFN avec un sulfate de polysaccharide réticulé et/ou un sulfate de polysaccharide immobilisé pour la fractionner en pFN et en cFN. Au moyen de la méthode de purification par fragmentation dévoilée, la cFN et la pFN peuvent être fractionnées rapidement et très efficacement, et peuvent être récupérées sous une forme très pure et avec un bon rendement.
Asakawa Kaneji
Hino Kazuo
Komai Takashi
Sakashita Eiji
Umemoto Ryoichi
Marks & Clerk
Otsuka Pharmaceutical Factory Inc.
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