Adsorption-desorption purification of glucose isomerase

C - Chemistry – Metallurgy – 12 – N

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C12N 9/92 (2006.01)

Patent

CA 1277625

ABSTRACT The present invention relates to a process for the production o a purified glucose isomerase which comprises contacting an impure extract containing glucose isomerase and soluble impurities with a weakly basic ion exchange material known to adsorb glucose isomerase; adding a first salt solution of a concentration which removes unadsorbed and weakly adsorbed impurities, but not glucose isomerase; and adding a second, buffered salt solution which elutes the adsorbed glucose isomerase.

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