Advanced active devices and methods for molecular biological...

C - Chemistry – Metallurgy – 12 – M

Patent

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Details

C12M 1/36 (2006.01) B01L 3/00 (2006.01) C12M 1/34 (2006.01) C12Q 1/68 (2006.01) C40B 60/00 (2006.01) H01L 21/58 (2006.01) H01L 25/00 (2006.01) H01L 27/00 (2006.01)

Patent

CA 2320798

Methods of manufacture and devices for performing active biological operations utilize various structures to advantageously collect and provide charged biological materials to an array of microlocations. In one embodiment, a device includes focusing electrodes to aid in the direction and transport of materials from a collection electrode to an array. Preferably, one or more intermediate transportation electrodes are utilized, most preferably of monotonically decreasing size between the collection electrode and the array, so as to reduce current density mismatches. In another aspect, a flow cell is utilized over devices to provide containment of solution containing materials to be analyzed. In yet another embodiment, a concentric ring design is provided. Various flip-chip embodiments are disclosed.

L'invention porte sur des procédés de fabrication et des dispositifs servant à effectuer à l'aide de différentes structures des opérations biologiques actives de recueil et de distribution de matériaux biologiques chargés sur un réseau de microsites. Dans l'une des réalisations, le dispositif comporte des électrodes focalisantes aidant à diriger et transférer des matériaux de l'électrode collectrice vers le réseau. On utilise de préférence une ou plusieurs électrodes intermédiaires de transfert, et mieux encore, des électrodes de taille décroissant monotonement, entre l'électrode collectrice et le réseau, pour réduire les disparités de densité de courant. Dans une autre réalisation, on utilise une cuve à circulation placée au-dessus des dispositifs pour retenir la solution contenant les matériaux à analyser. Dans une autre réalisation on utilise une structure à anneaux concentriques. L'invention porte également sur différentes exécutions à flip-chips.

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