Advanced selective plating media

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 1/20 (2006.01) C12N 1/00 (2006.01) C12Q 1/04 (2006.01)

Patent

CA 2651570

Selective growth media N4 agars that include a combination of high levels of yeast extract, possibly higher levels of protein, elevated levels of sugar and reduced levels of sodium chloride. The combination of ingredients provides the ability to detect Salmonella spp. and Shigella spp. with almost identical sensitivity while alleviating false-negative and false-positive problems commonly encountered with the presence of Proteus spp. and Citrobacter spp.

L'invention porte sur des milieux de culture sélectifs à base d'agar N4 qui comprennent une combinaison de niveaux élevés d'extraits de levures, de niveaux éventuellement supérieurs de protéines, de niveaux élevés de sucre et de niveaux réduits de chlorure de sodium. La combinaison d'ingrédients précitée permet de détecter Salmonella spp. et Shigella spp. avec une sensibilité presque identitique, tout en réduisant les problèmes de faux négatifs et de faux positifs généralement rencontrés en présence de Proteus spp. et de Citrobacter spp.

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