Aedes aegypti densovirus expression system

Details Aedes aegypti densovirus expression system Aedes aegypti densovirus expression system

C - Chemistry, Metallurgy

12

N

C12N 15/86 (2006.01) C12N 5/10 (2006.01) C12N 15/35 (2006.01) C12N 15/864 (2006.01) C12P 21/02 (2006.01)

Patent

CA 2204169

The present invention is directed to use of Aedes aegypti densonucleosis virus (Aedes DNV or AeDNV) as a heterologous gene expression system. The invention described herein provides promoters from the Aedes aegypti genome such as the p.05, p7 and p61 promoters to generate expression vectors with cloning sites for insertion of heterologous genes. The invention further provides expression vectors comprising an AeDNV promoter operably linked to a heterologous gene and insect host cells comprising the vectors of the invention. Methods of producing recombinant proteins utilizing the vectors of the invention are also provided. The present invention further provides a kit containing the vectors of the invention for production of a recombinant protein.

L'invention concerne l'utilisation du virus Aedes aegypti de la densonucléose (Aedes DNV ou AeDNV) comme système d'expression de gènes hétérologues. Elle concerne des promoteurs provenant du génome de Aedes aegypti, tels que p.05, p7 et p61, qui produisent des vecteurs d'expression dotés de sites de clonage permettant d'insérer des gènes hétérologues. L'invention concerne aussi des vecteurs d'expression qui comprennent des promoteurs d'AcDNV liés opérationnellement à un gène hétérologue, ainsi que des cellules hôtes d'insecte contenant les vecteurs propres à l'invention. On décrit aussi des procédés de production de protéines recombinées qui recourent à ces vecteurs. Cette invention concerne enfin une trousse contenant de tels vecteurs qui permettent de produire une protéine recombinée.

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