Aequorin as a reporter gene in yeast

Details Aequorin as a reporter gene in yeast Aequorin as a reporter gene in yeast

C - Chemistry, Metallurgy

12

Q

C12Q 1/02 (2006.01) C12N 1/19 (2006.01) C12N 15/81 (2006.01) C12Q 1/68 (2006.01) G01N 33/52 (2006.01)

Patent

CA 2464987

The invention concerns a modified yeast cell comprising an aequorin encoding sequence expressed under the control of the promoter of a mating factor- inducible gene. Moreover, it refers to a method of identofying compounds modulating the heterologous cell surface protein-mediated aequorin expression.

L'invention concerne une cellule de levure modifiée comprenant séquence codant pour une aequorine, dont l'expression est régulée par le promoteur d'un gène inductible par un facteur de conjugaison. L'invention concerne en outre un procédé permettant d'identifier des composés modulant l'expression d'aequorine régulée par une protéine de surface hétérologue.

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