Aeromonas salmonicida

A - Human Necessities – 61 – K

Patent

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Details

A61K 39/395 (2006.01) C07K 16/12 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2047388

ABSTRACT OF THE DISCLOSURE The present invention relates to a composition for passive immunization of fish against furunculosis caused by Aeromonas salmonicida, which comprises mammal immunoglobulins G specific to A. salmonicida in an aqueous solution. There is also provided a method for preparing a composition for passive immunization of fish against furunculosis caused by Aeromonas salmonicida, comprising the steps of: a) administering to a mammal selected from the group consisting of bovine, ovine, caprine and equine a whole culture of A. monicida to produce antibodies; b) collecting from the mammal the serum containing the mammal immunoglobulins G specific to A. salmonicida, c) precipitating and purifying said mammal immunoglobulins G by a single precipitation step with an aqueous ammonium sulphate solution in a concentration of about 40% weight/volume and separating the purified precipitated mammal immunoglobulins G; d) lyophilizing the purified mammal immunoglobulins G; and e) reconstituting the lyophilized mammal immunoglobulins G of step d) at about 5 to 7 x concentration in an intraperitoneal injectable carrier.

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