Affinity purification of polypeptide on protein a matrix

Details Affinity purification of polypeptide on protein a matrix Affinity purification of polypeptide on protein a matrix

C - Chemistry, Metallurgy

07

K

C07K 16/06 (2006.01)

Patent

CA 2271255

A method for purifying proteins by Protein A chromatography is described which comprises the steps of: (a) adsorbing the protein to Protein A immobilized on a solid phase comprising silica or glass; (b) removing contaminants bound to the solid phase by washing the solid phase with a hydrophobic electrolyte solvent; and (c) recovering the protein from the solid phase.

L'invention porte sur un procédé de purification de protéines par chromatographie de la protéine A, ce procédé consitant à: (a) faire adsorber la protéine par la protéine A immobilisée sur une phase solide comprenant de la silice ou du verre; (b) éliminier les contaminants liés à la phase solide par lavage de cette dernière avec un solvant d'électrolyte; et (c) récupérer la protéine dans la phase solide.

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