G - Physics – 01 – N
Patent
G - Physics
01
N
167/37
G01N 33/576 (2006.01)
Patent
CA 1113388
- 28 - Abstract of the Disclosure A test reagent for HBsAg is prepared by applying to a suitable substrate, such as a polystyrene bead, a first coat of purified HBsAg to an extent which will permit complete coverage by anti-HBs. After washing the HBsAg coated substrate serum containing anti-HBs, or an IgG fraction of serum containing anti-HBs, is applied as a second coat in an amount which substantially occupies the reacting groups of the HBsAg of the outside of the substrate and leaves an excess of antibody reacting groups in a free state, and any unattached material is washed off. A test sample which is to be tested for HBsAg is contacted with the reagent thus produced permitting attachment of HBsAg if present, and washing is done. Then a source of anti-HBs, such as an IgG fraction containing anti-HBs, which has been radio- actively labeled, is brought into contact with the reagent and any unattached material is washed off. The presence or absence of HBsAg in the test sample is determined from the presence or absence of significant additional radiation coming from the reagent as compared with a control. This method and its double coated test reagent permits securing high sensitivity without any manufacture of "dissociated" anti-HBs separately from the process itself, for use at any stage of the process, which separate manufacture of "dissociated" anti-HBs is a difficult and expensive procedure which adds greatly to the expense of any radioimmunoassay which calls for it to be done.
326938
Blumberg Baruch S.
Millman Irving
Borden Ladner Gervais Llp
Institute For Cancer Research (the)
LandOfFree
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