Agarose format for mammalian cell based reporter assays

G - Physics – 01 – N

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G01N 33/53 (2006.01) C12Q 1/00 (2006.01) C12Q 1/02 (2006.01) G01N 33/567 (2006.01)

Patent

CA 2339852

The present invention provides a novel method of using mammalian cells under a layer of agarose or embedded in agarose for transcription activation assays, where a functional response can be detected from the cells under a layer of agarose or embedded in agarose. The invention for the first time provides an agarose format for mammalian cell-based reporter assays useful in high throughput screening.

L'invention concerne un nouveau procédé d'utilisation de cellules mammaliennes recouvertes d'une couche d'agarose ou plongées dans l'agarose et servant dans des dosages d'activation par transcription. Selon ce procédé, une réponse fonctionnelle peut être détectée à partir des cellules recouvertes d'une couche d'agarose ou plongées dans l'agarose. Pour la première fois, cette invention propose un format d'agarose destiné aux dosages rapporteurs basés sur des cellules mammaliennes, utiles dans le criblage à haut rendement.

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