Agarose gel electrophoresis technique for the determination...

Details Agarose gel electrophoresis technique for the determination... Agarose gel electrophoresis technique for the determination...

C - Chemistry, Metallurgy

12

Q

324/23, 204/99.0

C12Q 1/40 (2006.01) C12Q 1/00 (2006.01) G01N 27/26 (2006.01) G01N 27/447 (2006.01)

Patent

CA 1210676

AN AGAROSE GEL ELECTROPHORESIS TECHNIQUE FOR THE DETERMINATION OF AMYLASE ISOENZYMES ABSTRACT OF THE DISCLOSURE Separation of all clinically relevant isoamylase bands including P1 P1b, P2, P3, P4, S1, S2, S3, S4 and S5 is achieved in a test in which a biological sample is electrophoresed on an agarose gel supporting a tris-sodium barbital-barbital buffer at a pH of between 8.4 and 9.2. The barbital anion concentration is between about 0.03 and about 0.08M, the tris cation concentration is between about 0.03M and about 0.07M; and the sodium cation concentration is between about 0.03M and about 0.07M. Agarose is present in the gel at concentrations of between about 0.4 and about 1.5 weight percent.

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