Agent for forming spheroids of hepatocytes and process for...

Details Agent for forming spheroids of hepatocytes and process for... Agent for forming spheroids of hepatocytes and process for...

C - Chemistry, Metallurgy

12

N

C12N 5/02 (2006.01) C08B 37/00 (2006.01) C08B 37/08 (2006.01) C12N 5/00 (2006.01) C12N 5/06 (2006.01)

Patent

CA 2077175

ABSTRACT OF THE DISCLOSURE An agent for the formation of spheroids of hepatocytes, which comprises a covalently lipid-bound glycosaminoglycan, and a culturing process for the formation of the spheroids. Hepatocytes spheroids can be formed by culturing hepatocytes in a culture vessel using a lipid-bound glycosaminoglycan as a culture substrate. Floating spheroids of hepatocytes can be obtained efficiently, which are capable of maintaining liver-specific functions and of keeping the spheroid form stably for a prolonged period of time. -72-

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