Agents for proliferating pancreatic beta-cells of the islets...

A - Human Necessities – 61 – K

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A61K 38/20 (2006.01) A61K 31/165 (2006.01) A61K 31/455 (2006.01) A61K 31/573 (2006.01) A61K 38/22 (2006.01) A61K 45/00 (2006.01) A61K 48/00 (2006.01) A61P 3/10 (2006.01) A61P 43/00 (2006.01) C07K 14/47 (2006.01) C07K 14/475 (2006.01) C12N 15/12 (2006.01) C12Q 1/02 (2006.01) G01N 33/15 (2006.01) G01N 33/50 (2006.01) C12N 5/06 (2006.01)

Patent

CA 2410918

It is indicated that the expression of Reg gene in .szlig. cells can be remarkably induced and thus the proliferation of .szlig. cells can be promoted by using an inflammation transmitter IL-6 together with glucocorticoid. It is also indicated that HGF inhibits apoptosis induced by Reg protein and that IL- 6 and glucocorticoid induce the expression of HGF gene in cells. PARP inhibitors such as nicotinic acid amide and 3-aminobenzamide further enhance the induction of the expression of these genes. Moreover, the expression induction mechanism is analyzed by using the promoter regions of Reg gene and HGF gene and a method of efficiently screening candidate compounds for these gene expression inducers is developed.

L'expression du gène Reg dans des cellules .beta. peut être remarquablement induite et partant, la prolifération desdites cellules peut être favorisée par l'utilisation d'un transmetteur d'inflammation IL-6 avec un glucocorticoïde. Il a également été découvert que HGF inhibe l'apoptose induite par la protéine Reg et que IL-6 et le glucocorticoïde induisent l'expression du gène HGF dans des cellules. Des inhibiteurs de PARP tels que l'amide d'acide nicotinique et le 3-aminobenzamide favorisent encore l'induction de l'expression de ces gènes. De plus, le mécanisme d'induction de l'expression est analysé à l'aide des régions promotrices du gène Reg et du gène HGF. La présente invention concerne enfin une méthode permettant de cribler efficacement des composés candidats pour ces inducteurs de l'expression génique.

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