Agglutination assays and kits employing colloidal dyes

G - Physics – 01 – N

Patent

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Details

G01N 33/546 (2006.01) G01N 33/543 (2006.01) G01N 33/558 (2006.01) G01N 33/574 (2006.01) G01N 33/58 (2006.01)

Patent

CA 2125769

The present invention provides methods and kits for determining the presence or amount of a substance by detection of a colloidal dye associated with agglutinated particles. The methods and kits are disclosed in direct and indirect (e.g., competitive) formats. In one aspect, the present invention provides methods and kits utilizing a single colloidal dye. In another aspect, methods and kits are provided which include two colloidal dyes, wherein one colloidal dye functions as a background-enhancing dye. A related aspect of the present invention provides methods and kits that include a colloidal dye and a non-water-soluble dye (which functions as a background-enhancing dye) attached to a microporous pad which permits differential migration of non-agglutinated and agglutinated latex particles. In yet another aspect, the present invention provides methods and kits utilizing a colloidal dye and a water-soluble dye, which functions as a background-enhancing dye.

Méthodes et nécessaires permettant de déterminer la présence ou la quantité d'une substance par la détection d'un colorant colloïdal associé aux particules agglutinées. Les méthodes et nécessaires sont présentés sous forme directe et indirecte (par exemple compétitive). Une des modalités de l'invention fait appel à un seul colorant colloïdal, une autre fait appel à deux colorants colloïdaux, l'un des deux servant à améliorer le contraste. Une autre modalité encore fait appel à un colorant colloïdal et à un colorant non soluble dans l'eau (servant à améliorer le contraste) contenu dans une compresse microporeuse et permettant la migration différentielle des particules de latex non agglutinées et les particules de latex agglutinées. Dans une autre modalité encore, la présente invention fournit des méthodes et des nécessaires faisant appel à un colorant colloïdal et à un colorant soluble dans l'eau servant à améliorer le contraste.

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