Agglutination immuno assay using two different particulate...

Details Agglutination immuno assay using two different particulate... Agglutination immuno assay using two different particulate...

G - Physics

01

N

167/37

G01N 33/543 (2006.01)

Patent

CA 1132902

ABSTRACT BIOLOGICAL ANALYSIS A particle agglutination assay for antigens, antibodies and other binding proteins such as rheumatoid factor, uses two different, microscopic or submicroscopic particulate reagents. The first particulate reagent binds with the antigen or antibody under assay, and then the second particulate reagent is added which binds only to those first reagent particles which have bound to the antigen or antibody under assay, so causing agglutination. The free unbound first or second particles are assayed to indicate the presence and/or amount of the antigen or antibody under assay. The assay of rheumatoid factor may be so conducted to reveal the fractions of each immunoglobulin class of RF present. The assay is part- icularly useful for small quantities of antibodies present in human sera indicating allergy, infection or autoimmune diseases.

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