Agglutrimetric assays in blood

Details Agglutrimetric assays in blood Agglutrimetric assays in blood

G - Physics

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N

G01N 33/567 (2006.01) C12Q 1/56 (2006.01) G01N 33/543 (2006.01) G01N 33/544 (2006.01) G01N 33/546 (2006.01)

Patent

CA 2284559

Methodology is provided for determining a characteristic of a sample, wherein problems associated with interference from whole blood, and oxygenated whole blood in particular, are avoided. Particularly, small particles are employed to which is bound a reagent, which interacts directly or indirectly, with a component related to the characteristic to be determined, which particles absorb in the infrared. By employing a protocol which results in the agglutination of the particles, the change in light absorption characteristics of the sample can be directly correlated with the presence, and as appropriate, function of the component related to the characteristic of interest in the sample.

Méthodes servant à déterminer une caractéristique d'un spécimen, ce qui permet d'éliminer les problèmes associés à l'interférence provenant du sang entier et, en particulier, du sang entier oxygéné. On utilise notamment de petites particules auxquelles est fixé un réactif exerçant une interaction directe ou indirecte avec un élément en relation avec la caractéristique à déterminer, ces particules absorbant la lumière infrarouge. La mise en application d'un protocole débouchant sur l'agglutination des particules permet de mettre en corrélation directe la modification des caractéristiques de l'absorption lumineuse du spécimen avec la présence et, si c'est le cas, la fonction de l'élément en relation avec la caractéristique recherchée du spécimen.

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