Aggregometer electrode structures

G - Physics – 01 – N

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G01N 33/48 (2006.01) G01N 27/07 (2006.01) G01N 33/49 (2006.01) G01N 33/84 (2006.01)

Patent

CA 1220523

17 ABSTRACT A disposable low cost electrode structure for measuring platelet aggregation in whole blood or PRP which utilizes con- ductive ink or foil for the electrodes with the electrode pat- tern printed, heat-stamped or silk-screened onto a suitable plastic non-reactive base. The base material can be any material which does not react with blood and is stiff enough, for ex- ample a polycarbonate, while the conductive element can be made with a silver conductive ink. The electrodes can be on one or both sides of the base or substrate material. Platelet aggregates start and build up on the leading edge of a narrow body inserted into the flow path of the blood. The conductive pattern must have its active element on or very near the leading edge of the electrode base material, and in contact with the blood, and the non-active areas of the conductive pattern must be insulated electrically from the blood or plasma being tested so as to allow sensing of the change in resistance caused by the platelet build-up on the active area. Tests of material thicknesses at stir- ring rates and in configurations that are used for aggrega- tion indicate that the platelets will build up on physical thicknesses ranging from less than 0.005 inches up to about 0.025 inches. Above that, the platelets, which average in diameter about 2 to 3 microns, do not accumulate on the edge. The electrodes should have a means for making con- nection to the measuring circuit which will sense platelet aggregation by measuring the increase of resistance between the two legs of the electrode assembly.

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