Agrobacterium-mediated plant transformation method

C - Chemistry – Metallurgy – 12 – N

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C12N 15/82 (2006.01) A01H 4/00 (2006.01) A01H 5/00 (2006.01)

Patent

CA 2381254

The present invention relates to a novel transformation system for generating transformed plants with lower copy inserts and improved transformation efficiency. In particular, the invention relates to the use of Agrobacterium growth inhibiting agents during the Agrobacterium-mediated transformation process that suppress Agrobacterium growth and reduce T-DNA transfer to the target plant genome.

L'invention concerne un nouveau système de transformation permettant de produire des plantes transformées en diminuant les insertions de copies et en améliorant l'efficacité de la transformation. L'invention concerne en particulier l'utilisation d'agents inhibant la croissance de l'agrobactérie pendant le processus de transformation par cette dernière; ces agents empêchant la croissance de l'agrobactérie et réduisant le transfert d'ADN-T vers le génome de la plante cible.

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