Agronomically elite soybeans with high beta-conglycinin content

A - Human Necessities – 01 – H

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A01H 5/00 (2006.01) A01H 5/10 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2621778

The invention overcomes the deficiencies of the art by providing an agronomically elite soybean plant with non-transgenic mutations of at least two of the glycinin subunits selected from the group consisting of GyI, Gy2, Gy3, Gy4, and Gy5, such as conferring a Gy 3 and Gy4 null phenotype and increased .beta.-conglycinin content in seed. The invention also provides derivatives, and plant parts of these plants and uses thereof. Methods for marker assisted selection of soybean varieties comprising non-transgenic mutations conferring a reduced GyI, Gy2, Gy3, Gy4, and Gy5 phenotype are also provided as part of the current invention. Methods for producing such plants that are further lipoxygenase and/or Kuiiitz Trypsin Inhibitor null and the plants produced thereby are also provided. The invention is significant in that soybeans from such plants are preferred dietary additives and provide important health benefits.

L'invention pallie les déficiences de l'état actuel de la technique au moyen d'une plante de soja de premier choix sur le plan agronomique comportant des mutations non transgéniques d'au moins deux des sous-unités de glycinine sélectionnées dans le groupe constitué de GyI, Gy2, Gy3, Gy4 et Gy5, de sorte que la semence reçoit un phénotype zéro par Gy 3 et Gy4, tout en présentant une teneur augmentée en ß-conglycinine. Elle concerne des dérivés et des parties de ces plantes et leurs utilisations. Elle concerne également des méthodes de sélection assistées par des marqueurs de variétés de soja comprenant des mutations non transgéniques conférant un phénotype limité par GyI, Gy2, Gy3, Gy4 et Gy5. Elle concerne, de plus, des méthodes servant à produire ces plantes qui sont également des inhibiteurs zéro de lipoxygénase et/ou de trypsine de Kuiiitz et les plantes obtenues au moyen de ces méthodes. Ces fèves de soja sont des additifs diététiques de qualité et présentent de nombreux avantages pour la santé.

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