Ahas mutants

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/54 (2006.01) A01H 5/00 (2006.01) A01H 5/10 (2006.01) A01N 25/32 (2006.01) C12N 15/82 (2006.01) C12Q 1/48 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2682349

The invention provides nucleic acids encoding mutants of the acetohydroxyacid synthase (AHAS) large subunit comprising at least two mutations, for example double and triple mutants, which are useful for producing transgenic or non-transgenic plants with improved levels of tolerance to AHAS-inhibiting herbicides. The invention also provides expression vectors, cells, plants comprising the polynucleotides encoding the AHAS large subunit double and triple mutants, plants comprising two or more AHAS large subunit single mutant polypeptides, and methods for making and using the same.

L'invention concerne des mutants de la grande sous-unité de l'acétohydroxyacide synthase(AHAS) encodant des acides nucléiques, comprenant au moins deux mutations, par exemple des mutants doubles ou triples, qui sont utiles pour la production de plantes transgéniques ou non transgéniques avec de meilleurs niveaux de tolérance aux herbicides inhibiteurs de l'AHAS. L'invention concerne également des vecteurs d'expression, des cellules et des plantes comprenant les polynucléotides encodant les mutants doubles ou triples de la grande sous unité de l'AHAS, des plantes comprenant deux polypeptides ou plus à simple mutation de la grande sous unité de l'AHAS, et les procédés pour leur fabrication et leur utilisation.

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