Airlift insect cell culture

Details Airlift insect cell culture Airlift insect cell culture

C - Chemistry, Metallurgy

12

N

195/40, 195/46

C12N 5/02 (2006.01) C12M 3/02 (2006.01)

Patent

CA 1309680

Abstract of the Disclosure Methods of growing insect cells in sparged culture, particularly in airlift fermentors to high cell densities with high viability at a large-scale, are herein disclosed. Further disclosed are media in which to grow airlift insect cell culture which media comprise a non-toxic protective agent, preferably a non-toxic water soluble polymer, and operating parameters, such as sparging rates and bubble sizes, that act to minimize cell damage and cell death from sparging. Further provided are methods and media for growing insect cells at large scale and for supporting the production therefrom of recombinant products when the cells are infected by recombinant baculoviruses, and viral products, when the cells are infected with wild-type viruses.

572554

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