Alcohol oxidase from pichia-type yeasts

Details Alcohol oxidase from pichia-type yeasts Alcohol oxidase from pichia-type yeasts

C - Chemistry, Metallurgy

12

N

324/28, 195/39

C12N 9/04 (2006.01)

Patent

CA 1193214

Abstract of the Disclosure A new alcohol oxidase is isolated from Pichia-type microorganisms in soluble or crystalline form. The crystalline novel enzyme is isolated by preparing an aqueous fluid containing cells of a Pichia-type microorganism, homogenizing the fluid and separating solids therefrom to produce an alcohol oxidase solution, adjusting the solution to have an ionic strength in the range of 0.05 to 0.01 in ionic strength to form a recovery range solution thereby causing the crystalline alcohol oxidase to form. The new enzyme is used to determine alcohol concentrations in fluid samples in which conditions are compatible with the enzyme's activity.

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