C - Chemistry – Metallurgy – 12 – P
Patent
C - Chemistry, Metallurgy
12
P
C12P 17/00 (2006.01) A23L 1/22 (2006.01) C07D 209/20 (2006.01) C12N 9/88 (2006.01)
Patent
CA 2643416
This invention relates to polypeptides having aldolase activity, including pyruvate activity such as, without limitation, HMG and/or KHG aldolase activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. In some embodiments, the invention is directed to polypeptides having aldolase activity, including pyruvate activity such as, without limitation, HMG and/or KHG aldolase activity, including thermostable and thermotolerant activity, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. The polypeptides in accordance with the invention can be used in a variety of pharmaceutical, agricultural and industrial contexts. In some embodiments, the invention provides polypeptides and biosynthetic pathways that are useful in the production of R-2-hydroxy 2-(indol-3ylmethyl)-4-keto glutaric acid (R-MP) and certain stereoisomers of monatin, such as R,R and S,R monatin, and salts thereof, as well as certain stereoisomers of monatin derivatives, such as the R3R and S5R configurations, and salts thereof.
L'invention concerne des polypeptides possédant une activité d'aldolase, comprenant une activité de pyruvate telle que l'activité d'aldolase HMG et/ou KHG, sans limitation, des polynucléotides codant ces polypeptides, et des procédés de fabrication et d'utilisation de ces polynucléotides et de ces polypeptides. Dans certains modes de réalisation, l'invention concerne des polypeptides possédant une activité d'aldolase, comprenant une activité de pyruvate telle que l'activité d'aldolase HMG et/ou KHG, sans limitation, ainsi qu'une activité thermostable et thermotolérante, et des polynucléotides codant ces enzymes, et la fabrication et l'utilisation de ces polynucléotides et de ces peptides. Les peptides de l'invention peuvent être utilisés dans une variété de contextes industriels, agricoles et pharmaceutiques. Dans certains modes de réalisation, l'invention concerne des polypeptides et des trajets biosynthétiques utiles dans la production d'acide glutarique de R-2-hydroxy 2-(indol-3ylméthyle)-4-céto (R-MP) et certains stéréoisomères de monatine, tels que la monatine R, R et S, R, et des sels de ceux-ci, ainsi que des stéréoisomères de dérivés de monatine, tels que des configurations R, R et S, R, et des sels de ceux-ci.
Burke Ellen
Gort Steven John
Hicks Paula M.
Luginbuhl Peter
Mcfarlan Sara C.
Cargill Incorporated
Sim & Mcburney
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