Alkaline protease

Details Alkaline protease Alkaline protease

C - Chemistry, Metallurgy

12

N

195/39

C12N 9/52 (2006.01) C12N 11/02 (2006.01) C12P 21/02 (2006.01)

Patent

CA 1204687

WO-2-31364C/KT/83. NOVEL ALKALINE PROTEASE ABSTRACT OF THE DISCLOSURE A novel protease is described. The enzyme has the following characteristics: molecular weight: 30,000 (a gel filtration method using Sephadex *G-75); isoelectric point, 5.3; pH reactivity: the optimum pH for its esterase activity is at pH 8.5 and the optimum pH for its amidase activity is at pH 9.0 respectively; substrate reactivity: it selectively and specifically hydrolyzes the ester bond and the amide bond at the carboxyl group of L-lysine; inhibitor: it is inhibited by diisopropyl phosphofluoride, tosyl-L-lysine chloro- methyl ketone and phenylmethylsulfonyl chloride. The protease is preferably produced by a microorganism belonging to the genus Achromobacter.

437655

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