Alkaline protease and preparation method thereof

Details Alkaline protease and preparation method thereof Alkaline protease and preparation method thereof

C - Chemistry, Metallurgy

12

N

195/128, 195/39

C12N 9/54 (2006.01)

Patent

CA 1193213

ABSTRACT OF THE DISCLOSURE Alkaline protease API-21 having the following physico- chemical properties: (1) Activity: Hydrolyzes casein under alkaline conditions, optimum pH range is 10 to 11, and has optimum temperature range for activity of 45°C to 50°C; (2) Stability: No decrease in enzymatic activity observed up to 40°C upon heating without substrate for 10 minutes at a pH of 10, but 90% or more decrease of enzymatic activity observed at 50°C for 10 minutes at a pH of 10. Deactivation occurs at 50°C for 10 minutes at a pH of 8 and complete deactivation substantially occurs at 60°C for 10 minutes at a pH of 8. The protease is stable under alkaline conditions, especially within a pH range of 7 to 11.5, and is stable against freezing and freeze-drying; and (3) Enzyme molecule: The protease is protein having a molecular weight of about 22000 as estimated by a gel filtration method, an isoelectric point of 7.4 as estimated by an electroforcusing method, an absorption peak of 275 to 282 nm in a UV absorption spectrum, IR absorption characteristics as shown in Figure 5, and a serine residue is the active site thereof. This alkaline protease is prepared by cultivating novel species of NKS-21 belonging to genus Bacillus.

420937

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