C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 9/54 (2006.01) C11D 3/386 (2006.01) C11D 7/42 (2006.01) C12N 1/20 (2006.01)
Patent
CA 2066556
ABSTRACT An alkaline protease, method for producing the same and detergents containing the protease as an aid, the alkaline protease obtained by cultivating in a medium a microorganism SD523 FERM BP-3774 and having an ability of producing the alkaline protease, or artificial mutants or genetically engineered variants thereof, the microorganism having (1) an optimum pH of from about 11 to 11.5 as measured after reaction at 30°C for 10 minutes using casein as a substrate and an optimum temperature of about 60°C as measured after reaction at pH 10 using casein as a substrate; (2) a half inactivation temperature of about 55°C as measured after reaction at pH 10 for 10 minutes; and (3) a molecular weight of 29,000 ? 2,000 as measured by SDS polyacrylamide gel electrophoresis. The enzyme has an improved storage stability in liquid detergents as compared with known alkaline proteases produced by known microorganisms belonging to the genus Bacillus firmus.
Fujiwara Yoshio
Noguchi Yoshitaka
Saito Mina
Sawayanagi Toyoji
Tsuzuki Toshi
Novo Nordisk A/s
Sim & Mcburney
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