Allergen detection

C - Chemistry – Metallurgy – 12 – Q

Patent

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Details

C12Q 1/37 (2006.01) G01N 33/52 (2006.01) G01N 33/58 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2376500

A method of determining allergen activity in dust comprises: providing a dust sample; extracting from the dust sample at least one breakdown component or proteins or peptides; reacting the extracted at least one breakdown com-ponent with a colorimetric amine detection reagent such as TNBSA; and quantitatively measuring the intensity of any re-sulting coloration. The allergen activity may be gauged by the intensity of coloration. Image

L'invention concerne un procédé destiné à déterminer l'activité des allergènes se trouvant dans la poussière. Ce procédé consiste à prélever un échantillon de poussière, à extraire au moins un produit de décomposition de protéines ou de peptides à partir dudit échantillon de poussière, à faire réagir ce produit de décomposition avec un réactif de détection d'amine colorimétrique, tel que le TNBSA, et à mesurer quantitativement l'intensité de la coloration qui en résulte. L'activité des allergènes peut être mesurée en fonction de l'intensité de la coloration.

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