Allergen-microarray assay

Details Allergen-microarray assay Allergen-microarray assay

G - Physics

01

N

G01N 33/68 (2006.01)

Patent

CA 2424661

A method is provided for the detection of an immunoglobulin which binds to an allergen in a sample, whereby one or more allergens are immobilized on a microarray chip after which the sample is incubated with the immobilized allergens so that immunoglobulins which are specific for the allergens bind to the specific allergen after which the immunoglobulins which are bound to the specific immobilized allergens are detected, as well as a method for in vitro diagnosis of allergies in a patient.

L'invention concerne un procédé relatif à la détection d'une immunoglobuline qui se lie avec un allergène dans un échantillon. On immobilise un ou plusieurs allergènes dans un jeu ordonné de microéchantillons, puis l'échantillon est incubé avec ces allergènes immobilisés pour que les immunoglobulines spécifiques aux allergènes se lient avec un allergène spécifique. On détecte ensuite les immunoglobulines liées aux allergènes spécifiques immobilisés. L'invention concerne en outre un procédé de diagnostic in vitro des allergies chez un patient.

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