Alloantigen enhancement assay

G - Physics – 01 – N

Patent

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Details

G01N 33/577 (2006.01) G01N 33/564 (2006.01) G01N 33/569 (2006.01) G01N 33/80 (2006.01)

Patent

CA 2145759

Methods and compositions are provided for the determination of cross-reactive alleles, where there is no antibody which will specifically distinguish between the two alleles. Particularly, the method employs an antibody which binds to the two alleles bound to a surface, an antibody specific for one of the alleles, a labeled conjugate which binds to a consensus sequence present in both alleles and positive and negative controls. By having an enhanced value where the interfering allele is present as compared to a value for the target allele, one can distinguish between the various alternatives involving the presence or absence of one or both alleles.

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