Allografts combined with tissue derived stem cells for bone...

A - Human Necessities – 61 – L

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A61L 27/12 (2006.01) A61K 47/02 (2006.01) A61L 27/38 (2006.01)

Patent

CA 2743869

There is disclosed a method of combining mesenchymal stem cells (MSCs) with a bone substrate. In an embodiment, the method includes obtaining tissue having MSCs together with unwanted cells. The tissue is digested to form a cell suspension having MSCs and un-wanted cells. The cell suspension is added to the sub-strate. The substrate is cultured to allow the MSCs to adhere. The substrate is rinsed to remove unwanted cells. In various embodiments, the tissue is adipose issue, muscle tissue, or bone marrow tissue. In an embodiment, there is disclosed an allograft product including a combination of MSCs with a bone substrate in which the combination is manufactured by culturing MSCs disposed on the sub-strate for a period of time to allow the MSCs to adhere to the substrate, and then rinsing the substrate to remove un-wanted cells from the substrate. Other embodiments are also disclosed.

La présente invention concerne un procédé de combinaison de cellules souches mésenchymateuses (MSC) avec un substrat osseux. Dans un mode de réalisation, le procédé comprend l'obtention de tissu ayant des MSC conjointement avec des cellules indésirables. Le tissu est digéré pour former une suspension de cellules comprenant des MSC et des cellules indésirables. La suspension de cellules est ajoutée au substrat. Le substrat est cultivé de manière à permettre aux MSC d'adhérer. Le substrat est rincé afin d'éliminer les cellules indésirables. Dans différents modes de réalisation, le tissu est un tissu adipeux, un tissu musculaire, ou un tissu de moelle osseuse. Dans un mode de réalisation, il est décrit un produit d'allogreffe comprenant une combinaison de MSC avec un substrat osseux où la combinaison est produite par culture de MSC disposées sur le substrat pendant un certain temps pour permettre aux MSC d'adhérer au substrat, puis rinçage du substrat pour enlever les cellules indésirables du substrat. D'autres modes de réalisation sont également décrits.

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