Alpha 1,2-fucosyltransferase from helicobacter pylori

C - Chemistry – Metallurgy – 12 – N

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C12N 15/54 (2006.01) C07K 16/40 (2006.01) C12N 9/10 (2006.01) C12P 19/18 (2006.01) C12Q 1/48 (2006.01) C12Q 1/68 (2006.01) G01N 33/569 (2006.01) G01N 33/574 (2006.01)

Patent

CA 2349428

A bacterial .alpha. 1,2 fucosyltransferase gene and deduced amino acid sequence is provided. The gene is useful for preparing .alpha. 1,2-fucosyltransferase polypeptide, and active fragment thereof, which can be used in the production of oligosaccharides such as Lewis X, Lewis Y, Lewis B and H type 1, which are structurally similar to certain tumor-associated carbohydrate antigens found in mammals. These product glycoconjugates also have research and diagnostic utility in the development of assays to detect mammalian tumors.

L'invention concerne un gène bactérien alpha 1,2-fucosyltransférase et une séquence d'aminoacides déduite. Le gène sert à préparer un polypeptide alpha 1,2-fucosyltransférase et son fragment actif qui peut être utilisé dans la production d'oligosaccharides tels que Lewis X, Lewis Y, Lewis B et H type 1, qui sont structurellement semblables à certains antigènes de carbohydrate associés à une tumeur, antigènes trouvés chez les mammifères. Ces glycoconjugués obtenus présentent un intérêt en termes de recherche et de diagnostic pour la mise au point d'essais permettant de déceler des tumeurs chez les mammifères.

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