.alpha.1,3-fucosyltransferase nucleic acid and polypeptide

Details .alpha.1,3-fucosyltransferase nucleic acid and polypeptide .alpha.1,3-fucosyltransferase nucleic acid and polypeptide

C - Chemistry, Metallurgy

12

N

C12N 15/54 (2006.01) A01H 1/00 (2006.01) A01H 5/00 (2006.01) A01K 67/027 (2006.01) A61K 38/45 (2006.01) A61K 48/00 (2006.01) C07K 16/40 (2006.01) C12N 1/21 (2006.01) C12N 5/10 (2006.01) C12N 9/10 (2006.01) C12N 15/09 (2006.01) C12N 15/63 (2006.01) C12N 15/67 (2006.01) C12P 19/04 (2006.01) C12P 19/26 (2006.01) C12P 21/00 (2006.01) C12P 21/02 (2006.01) C12Q 1/02 (2006.01) C12Q 1/48 (2006.01) C12Q 1/68 (2006.01) G01N 33/53 (2006.01) G01N 33/566 (2006.01)

Patent

CA 2338981

A novel polypeptide having an .alpha.1,3-fucosyltransferase activity; a process for producing this polypeptide; a DNA encoding this polypeptide; a process for producing this DNA; a recombinant vector obtained by integrating this DNA; a transformant carrying this recombinant vector; an antibody recognizing the above polypeptide; a method for quantitating the above polypeptide and an immunostaining method by using this antibody; a process for producing a fucose-containing sugar chain by using the above polypeptide or the above transformant; a method for screening a substance altering the expression of a gene encoding the above polypeptide; a method for screening a substance altering the activity of the polypeptide; and a method for diagnosing brain diseases, renal diseases, cancer, etc. by using the above DNA or the above antibody.

L'invention concerne: un nouveau polypeptide présentant l'activité d'une alpha 1,3-fucosyltransférase; un procédé de production dudit polypeptide; un ADN codant ce polypeptide; un procédé de production de cet ADN; un vecteur recombiné obtenu par intégration de cet ADN; un transformant comportant ce vecteur recombiné; un anticorps reconnaissant ledit polypeptide; un procédé pour quantifier ledit polypeptide et une méthode d'immunocoloration faisant appel à cet anticorps; un procédé pour produire une chaîne sucre contenant du fucose, au moyen dudit polypeptide ou dudit transformant; un procédé pour cribler une substance modifiant l'expression d'un gène codant ledit polypeptide; un procédé pour cribler une substance modifiant l'activité dudit polypeptide; et un procédé pour diagnostiquer des affections cérébrales, des maladies des reins, le cancer, etc. au moyen dudit ADN ou dudit anticorps.

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