.alpha.1-3 galactosyltransferase gene and promoter

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/54 (2006.01) A01K 67/027 (2006.01) C12N 5/10 (2006.01) C12N 9/10 (2006.01) C12N 15/85 (2006.01)

Patent

CA 2426669

The present invention provides a recombinant expression cassette comprising an .alpha. 1-3 galactosyltransferase promoter operably linked to a polynucleotide for expression. The invention also provides a recombinant mutating cassette comprising a region of homology to an .alpha. 1-3 galactosyltransferase genomic sequence. The cassettes can be employed to express foreign genes or to disrupt the native .alpha. 1-3 galactosyltransferase genomic sequence, particularly within an animal. Thus, the invention also provides transgenic animals and methods for their production and use.

Cette invention a trait à une cassette d'expression de recombinaison comprenant un promoteur de galactosyltransférase-.alpha.-1-3 lié fonctionnellement à un polynucléotide aux fins d'expression. L'invention concerne également une cassette de recombinaison mutante possédant une région d'homologie avec une séquence génomique de galactosyltransférase-.alpha.-1-3. On peut utiliser ces cassettes pour exprimer des gènes étrangers ou pour disloquer la séquence génomique de galactosyltransférase-.alpha.-1-3 endogène, notamment chez l'animal. Cette invention porte ainsi sur des animaux transgéniques ainsi que sur des procédés permettant de les produire et de les utiliser.

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