.alpha.-1,4-glucan lyase and its use in the production of...

Details .alpha.-1,4-glucan lyase and its use in the production of... .alpha.-1,4-glucan lyase and its use in the production of...

C - Chemistry, Metallurgy

12

N

C12N 15/60 (2006.01) A23L 1/236 (2006.01) A23L 3/3562 (2006.01) C12N 9/88 (2006.01) C12N 9/96 (2006.01) C12P 19/02 (2006.01)

Patent

CA 2202374

An enzyme isolatable from algae is described. Also, a method of preparing the sugar 1,5-D-anhydrofructose is described. The method comprises treating an .alpha.-1,4-glucan with an .alpha.-1,4-glucan lyase wherein the enzyme is used in substantially pure form. In a preferred embodiment, if the glucan contains links other than and in addition to the .alpha.-1,4-links, the .alpha.-1,4- glucan lyase is used in conjunction with a suitable reagent that can break the other links.

On décrit une enzyme que l'on peut isoler à partir d'algues, ainsi qu'un procédé de préparation du 1,5-D-anhydrofructose du sucre. Ce procédé consiste à traiter un .alpha.-1,4-glucane avec une .alpha.-1,4-glucane lyase, cette enzyme étant utilisée sous une forme sensiblement pure. Dans un mode préféré de réalisation, si le glucane contient des liaisons autres que les liaisons .alpha.-1,4- et s'ajoutant à ces dernières, on utilise l'.alpha.-1,4-glucane lyase conjointement avec un réactif approprié capable de rompre ces autres liaisons.

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