C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
252/23, 195/1.23
C12N 9/02 (2006.01) C12N 9/80 (2006.01) C12N 11/00 (2006.01) C12N 15/67 (2006.01)
Patent
CA 1341454
Purified enzymatic compositions are provided having alpha-amidating enzymes capable of catalyzing the conversion of a peptidyl compound having a C-terminal glycine residue to a corresponding peptidyl amide having an amino group in place of the C-terminal glycine. The purified compositions have specific activities above 25mU per mg protein and are sufficiently free of proteases to allow effective catalysis of even peptidyl compounds having L-amino acids. Biologically important alpha-amidated products such as calcitonin and other regulatory hormones are efficiently produced using the alpha-amidation reaction catalyzed by the enzymes. Purification by size exclusion chromatography in combination with strong anion exchange chromatography results in homogeneous enzyme species which are used to prepare antibodies specific for the alpha-amidating enzyme. A gene capable of expressing the alpha-amidating enzyme is ligated into an expression vector and transformed into a host cell capable of expressing the gene.
574685
Bertelsen Arthur H.
Gilligan James P.
Jones Barry N.
Mehta Nozer M.
Bertelsen Arthur H.
Gilligan James P.
Jones Barry N.
Marks & Clerk
Mehta Nozer M.
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