.alpha. amylase assay

Details .alpha. amylase assay .alpha. amylase assay

C - Chemistry, Metallurgy

12

Q

150/15.2, 150/3.

C12Q 1/40 (2006.01)

Patent

CA 1260369

Ab?tract of the Disclosure Method of measuring the amount of .alpha. -amylase in a liquid sample, including the steps of providing an oligosaccharide substrate for .alpha.-amylase, the substrate being characterized in that it contains at least 3 glucose units, its reducing-end glucose unit is bonded, via a bond cleavable by .alpha.-or .beta. -glucosidase, to a label which exhibits an optically measurable change upon cleavage of the bond, and its terminal glucose unit is bonded to a blocking substituent which inhibits cleavage by exo-enzymes of the bond between the terminal glucose unit and the adjacent glucose unit; contacting the sample with the oligosaccharide substrate and with a first exo-enzyme capable of cleaving the bond between the reducing-end glucose unit and the label, and measuring the optically measurable change as a measure of .alpha. -amylase in the sample.

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