.alpha.-amylase mutants

Details .alpha.-amylase mutants .alpha.-amylase mutants

C - Chemistry, Metallurgy

12

N

C12N 9/26 (2006.01) C11D 3/386 (2006.01) C12N 9/28 (2006.01) C12N 15/00 (2006.01) C12N 15/56 (2006.01) C12S 11/00 (2006.01)

Patent

CA 2745783

The invention relates to a variant of a parent Termamyl-like .alpha.-amylase, comprising mutations in two, three, four, five or six regions/positions. The variants have increased thermostability at acidic pH and/or at low Ca2+ concentrations (relative to the parent). The invention also relates to a DNA construct comprising a DNA sequence encoding an .alpha.-amylase variant of the invention, a recombinant expression vector which carries a DNA construct of the invention, a cell which is transformed with a DNA construct of the invention, the use of an .alpha.- amylase variant of the invention for washing and/or dishwashing, textile desizing, starch liquefaction, a detergent additive comprising an .alpha.-amylase variant of the invention, a manual or automatic dishwashing detergent composition comprising an .alpha.-amylase variant of the invention, a method for generating a variant of a parent Termamyl-like .alpha.-amylase, which variant exhibits increased thermostability at acidic pH and/or at low Ca2+ concentrations (relative to the parent).

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