.alpha.-amylase variants

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 9/28 (2006.01) C11D 3/386 (2006.01) C12N 1/21 (2006.01) C12N 9/96 (2006.01) C12N 15/01 (2006.01) C12N 15/09 (2006.01)

Patent

CA 2350837

The invention relates to a variant of a parent Termamyl-like .alpha.-amylase, comprising mutations in two, three, four, five or six regions/positions. The variants have increased stability at high temperatures (relative to the parent). The invention also relates to a DNA construct comprising a DNA sequence encoding an .alpha.-amylase variant of the invention, a recombinant expression vector which carries a DNA construct of the invention, a cell which is transformed with a DNA construct of the invention, the use of an .alpha.- amylase variant of the invention for washing and/or dishwashing, textile desizing, starch liquefaction, a detergent additive comprising an .alpha.- amylase variant of the invention, a manual or automatic dishwashing detergent composition comprising an .alpha.-amylase variant of the invention, a method for generating a variant of a parent Termamyl-like .alpha.-amylase, which variant exhibits increased.

L'invention concerne une variante d'un parent d'.alpha.-amylase de type Termamyle qui comprend des mutations dans les régions/positions deux, trois, quatre, cinq ou six. Les variantes possèdent une plus grande stabilité à des températures élevées que le parent. L'invention concerne aussi à une construction d'ADN comprenant une séquence d'ADN codant pour une variante d'.alpha.-amylase de l'invention, un vecteur d'expression recombinant qui transporte une construction d'ADN de l'invention, une cellule transformée au moyen de la construction d'ADN de l'invention, l'utilisation de la variante d'.alpha.-amylase de l'invention dans le lavage et/ou le lavage de vaisselle, dans le désencollage de textiles, la liquéfaction d'amidon ainsi qu'un additif pour détergents comprenant une variante d'.alpha.-amylase de l'invention, une composition détergente pour lavage à la main ou en machine qui comprend une variante d'.alpha.-amylase de l'invention, un procédé pour générer une variante d'un parent d'.alpha.-amylase de type Termamyle, ladite variante manifestant des propriétés améliorées.

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